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Article Name The Effects of Allogenic Mesenchymal Stem Cells on Healing of Fractures in The Bone Undergone Necrosis
Author Name Fırat OZAN*, Mevlüt Hakan DİNÇ**, Mahmut PEKEDİS***, Hasan YILDIZ***, Osman Arslan BORA**
Address * İzmir Bozyaka Eğitim ve Araştırma Hastanesi Ortopedi ve Travmatoloji Kliniği, İzmir/TÜRKİYE ** İzmir Atatürk Eğitim ve Araştırma Hastanesi II. Ortopedi ve Travmatoloji Kliniği, İzmir/TÜRKİYE *** Ege Üniversitesi Mühendislik Fakültesi Makine Mühendisliği Bölümü, İzmir/TÜRKİYE
Abstract In this study the contributions of allogenic mesenchymal stem cells, injected into rat femur fracture directly, to the fusion of fractures on bone undergone necrosis and the reformation of the bone were investigated. A total of 31 Wistar Albino rats were used in this study. Allogenic mesenchymal stem cells were obtained from seven rats. Remaining rats were divided into two groups involving 12 rats in each. Group 1 (n=12); a femur fracture was formed, medulla of femur washed with formalin of 10 %, intramedullary fixation was practiced and stem cell was applied to fracture line. Group 2 (n=12) comprising control group; a femur fracture was formed, medulla of femur washed with formalin of 10 % and intramedullary fixation was practiced. At the end of study healing at the broken femurs of the rats sacrificed was evaluated clinically, radiologically and biomechanically. At the end of study the fractures on seven femurs in group 1 and on eight femurs in group 2 were manually examined but no pathological movement was observed. Radiologically, a statistically significant difference was not detected between groups (P=0.149). A statistically significant difference was not detected between groups in terms of metafizodiafizer area and callus area measurements (P=0.52). When they are examined biomechanically a statistically significant difference was not found between their stiffness, elasticity and energy storage capacities respectively (P=0.22, P=0.06, P=0.56). We consider that mesenchymal stem cells can not demonstrate osteogenic features without appropriate osteoinductive, osteoconductive and efficient blood flow.
Published in Electronic Journal of BioTechnology
Issue 0
Volume 2
Pages 1-9
Year 2010
Type Paper
Language Turkish
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